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accessory plasmid ptofa  (TaKaRa)


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    TaKaRa accessory plasmid ptofa
    Accessory Plasmid Ptofa, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmid/pm37944824-174-26-30
    Average 86 stars, based on 1 article reviews
    accessory plasmid ptofa - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Chelerythrine chloride inhibits Zika virus infection by targeting the viral NS4B protein.
    Article Snippet: Zika virus (ZIKV) is a mosquito-borne virus that has re-emerged as a significant threat to global health in the recent decade.. Whilst infections are primarily asymptomatic, the virus has been associated with the manifestation of severe neurological complications.. At present, there is still a lack of approved antivirals for ZIKV infections.

    Transfection:

    Article Title: Chelerythrine chloride inhibits Zika virus infection by targeting the viral NS4B protein.
    Article Snippet: Zika virus (ZIKV) is a mosquito-borne virus that has re-emerged as a significant threat to global health in the recent decade.. Whilst infections are primarily asymptomatic, the virus has been associated with the manifestation of severe neurological complications.. At present, there is still a lack of approved antivirals for ZIKV infections.



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    Image Search Results


    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 expression. A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression vectors and then lysed for protein detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.

    Journal: ACS Omega

    Article Title: SARS-CoV-2 Protein Nsp9 Is Involved in Viral Evasion through Interactions with Innate Immune Pathways

    doi: 10.1021/acsomega.4c02631

    Figure Lengend Snippet: SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 expression. A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression vectors and then lysed for protein detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.

    Article Snippet: SARS-CoV-2 nonstructural and accessory protein expression vectors were purchased from Addgene (MA, USA): pTwist-CMV-Nsp9–3xFlag (pGBW- m4133209), pTwist-CMV-ORF6–3xFlag (pGBW-m4252831), and pTwist-CMV-ORF3a- 3xFlag (pGBW-m4133902).

    Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, Control